mouse monoclonal anti emerin  (Santa Cruz Biotechnology)

Journal: Advanced Science

Article Title: Mechanical Stress Triggers Premature Senescence in Cardiac Fibroblasts

doi: 10.1002/advs.202513314

Figure Lengend Snippet: Mechanosensitive nuclear envelope protein, emerin, may be a key mediator in the initiation of a mechanically‐induced senescence phenotype in cardiac fibroblasts. Representative images show emerin staining for nuclei from WT CFs from mechanically‐induced, chemically‐induced, and respective control cultures. Scale bar = 10 µm. A) Quantifying the emerin ring thickness in Stretch Control and Stretch Injury CFs indicated a decreased ring in Stretch Injury CFs. N = 5 animals. n ≥ 25 nuclei/treatment. B) Emerin ring thickness between TCP and ROS‐induced CFs showed no significant alterations with the induction of a senescent phenotype. N = 6–8 animals, n ≥ 25 nuclei/treatment. Error bar = 1 Std. Linear mixed model, ANOVA. **** p < 0.0001.

Article Snippet: Primary Antibodies: Mouse anti‐Mouse γH2A.X (1:400, CST, 80312S), Rabbit anti‐Mouse Lamin B1 (1:500, abcam, ab16048), Rat anti‐Mouse Ki‐67 (1:300, ebiosciences, 14‐5698‐82), Rabbit anti‐Mouse Emerin (1:250, CST, 30853S).

Techniques: Staining, Control

Journal: bioRxiv

Article Title: Perturbed cyclic strain results in a mechanically-induced, premature senescent phenotype in cardiac fibroblasts

doi: 10.1101/2025.03.16.643588

Figure Lengend Snippet: Representative images show emerin staining for nuclei from WT CFs from mechanically-induced, chemically-induced, and respective control cultures. Scale bar = 10µm. (A) Quantifying the emerin ring thickness in Stretch Control and Stretch Injury CFs indicated a decreased ring in Stretch Injury CFs. N = 5 animals. (B) Emerin ring thickness between TCP and ROS-induced CFs showed no significant alterations with the induction of a senescent phenotype. N = 6-8 animals, n ≥ 25 nuclei/treatment. Error bar = 1 Std. Linear mixed model, ANOVA. ****p<0.0001.

Article Snippet: Primary Antibodies: Mouse anti-Mouse ɣH2A.X (1:400, CST, 80312S), Rabbit anti-Mouse Lamin B1 (1:500, abcam, ab16048), Rat anti-Mouse Ki-67 (1:300, ebiosciences, 14-5698-82), Rabbit anti-Mouse Emerin (1:250, CST, 30853S).

Techniques: Staining, Control

Journal: bioRxiv

Article Title: Perturbed cyclic strain results in a mechanically-induced, premature senescent phenotype in cardiac fibroblasts

doi: 10.1101/2025.03.16.643588

Figure Lengend Snippet: (A) Quantified emerin ring thickness was similar in Stretch Control and Stretch Injury lamin A/C null CFs. N = 5 animals. n ≥ 25 nuclei/treatment, Error bar = 1 Std. (B) No difference in quantified emerin ring thickness was observed in lamin A/C null CFs on TCP or treated with hydrogen peroxide. N = 5-7 animals, n ≥ 25 nuclei/treatment, Error bar = 1 Std.

Article Snippet: Primary Antibodies: Mouse anti-Mouse ɣH2A.X (1:400, CST, 80312S), Rabbit anti-Mouse Lamin B1 (1:500, abcam, ab16048), Rat anti-Mouse Ki-67 (1:300, ebiosciences, 14-5698-82), Rabbit anti-Mouse Emerin (1:250, CST, 30853S).

Techniques: Control